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xtt proliferation kit ka1387  (Abnova)
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Abnova xtt proliferation kit ka1387
Treatment with increasing doses of BMAA resulted in decreased cell number and inhibition of cell <t>proliferation</t> as measured by <t>XTT</t> assay . Although proliferative inhibition trended with increased BMAA dose, statistical significance was not observed at concentrations less than 4uM . These findings support the hypothesis that while BMAA may be capable of exhibiting repression of proliferative cells, it may cause cellular defects via additional processes, especially at low concentrations.
Xtt Proliferation Kit Ka1387, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Treatment with increasing doses of BMAA resulted in decreased cell number and inhibition of cell proliferation as measured by XTT assay . Although proliferative inhibition trended with increased BMAA dose, statistical significance was not observed at concentrations less than 4uM . These findings support the hypothesis that while BMAA may be capable of exhibiting repression of proliferative cells, it may cause cellular defects via additional processes, especially at low concentrations.

Journal: bioRxiv

Article Title: The biotoxin BMAA promotes dysfunction via distinct mechanisms in neuroblastoma and glioblastoma cells

doi: 10.1101/2022.11.24.517806

Figure Lengend Snippet: Treatment with increasing doses of BMAA resulted in decreased cell number and inhibition of cell proliferation as measured by XTT assay . Although proliferative inhibition trended with increased BMAA dose, statistical significance was not observed at concentrations less than 4uM . These findings support the hypothesis that while BMAA may be capable of exhibiting repression of proliferative cells, it may cause cellular defects via additional processes, especially at low concentrations.

Article Snippet: Cell proliferation was quantified using the XTT proliferation kit (Abnova Cat. No. KA1387) according to manufacturer’s instructions.

Techniques: Inhibition, XTT Assay

Cell number is enhanced in the glioblastoma cell line U118MG by BMAA treatment for 7 days (A). XTT assay shows BMAA induces proliferation over a 7 day incubation period (B). BMAA does not increase cytotoxicity in U118MG cells, even at 25uM doses. Cell lysis in response to BMAA was measured by a fluorescence-based LDH cytotoxicity assay (C).

Journal: bioRxiv

Article Title: The biotoxin BMAA promotes dysfunction via distinct mechanisms in neuroblastoma and glioblastoma cells

doi: 10.1101/2022.11.24.517806

Figure Lengend Snippet: Cell number is enhanced in the glioblastoma cell line U118MG by BMAA treatment for 7 days (A). XTT assay shows BMAA induces proliferation over a 7 day incubation period (B). BMAA does not increase cytotoxicity in U118MG cells, even at 25uM doses. Cell lysis in response to BMAA was measured by a fluorescence-based LDH cytotoxicity assay (C).

Article Snippet: Cell proliferation was quantified using the XTT proliferation kit (Abnova Cat. No. KA1387) according to manufacturer’s instructions.

Techniques: XTT Assay, Incubation, Lysis, Fluorescence, LDH Cytotoxicity Assay